Dual-energy X-ray Absorptiometry

Location : Building A, Semi-SPF Shared Laboratory

How to use

1. Turn on the computer.
2. Turn on Maestro and wait about 1 minute for initialization.
3. If the focus Knob is connected, check if the blue light is on. If it is not on, check the 3D Connexion Control panel on the computer.
4. Launch Maestro software. (There is no need to wait for the LCTF to initialize.)
5. Press the Excitation Lamp button for 3 seconds to turn on the excitation lamp.

Manual

Hematology Analyzer

Location : Building A, Semi-SPF Shared Laboratory

How to use

1. Turn on the power by pressing the ON button on the back of the machine.
2. Log in by entering ID: Admin PW: Admin on the screen. (Press the Caps Lock button once and enter A, then press the Caps Lock button again to switch to lowercase and enter dmin)
3. Enter notes in QC-> Set up in the Menu. (QC-> Count is for taking photos after the experiment.)
4. Mix the collected blood well and insert it into the analyzer's machine.
5. Results can be reviewed by sending them to a printout or a computer.

Manual

Biochemical Analyzer

Location : Building A, Semi-SPF Shared Laboratory

How to use

1. Turn on the power by pressing the ON button on the right side of the machine.
2. Each test requires 10μL of sample. (CRP requires 5μL/test and ISE requires 50μL/3 tests)
3. Set the slides and specimens, insert the tips (thinner and more pointed than micro tubes), and press the start key. (All subsequent processes are fully automated)
4. Up to 5 specimens can be set at the same time, and there is no manual operation after pressing the start key.
5. Corrections can be made by inserting the QC card into the main unit.

Manual Instructions

Cell/tissue Incubator

Location : Building A, Clean Lab 2

How to use

1. Plug in the device.
2. Disinfect the interior of the equipment by spraying alcohol and wiping it with paper towels.
3. Depending on the experiment, add distilled water to the wide tray at the bottom
4. Open the CO2 gas tank, set the temperature and CO2 concentration, and wait until the set environment is reached.
5. After the incubation is finished, disinfect the interior of the equipment by spraying alcohol and wiping it with paper towels, close the CO2 gas tank, and unplug the device.

Manual

Inhalation Anesthesia System

Location : Building A (Semi-SPF), Building B (Conventional)

How to use

1. Place the animal in the chamber (box).
2. Open the oxygen tank gas valve, fill with an appropriate amount of isoflurane, and open the anesthetic lever.
3. Once the animal is anesthetized, remove it from the chamber, place the animal's face into the hose, and turn the orange valve, which was directing gas toward the chamber, toward the hose.
4. After use, close the anesthetic and oxygen tank valves, open the inhalation anesthesia machine's oxygen valve to remove residual pressure, and then close it completely.

In Vivo Imaging System

Location : Building A, Clean Lab 1

Inquiry about equipment : Equipment Center Representative 02-3290-4064

Instructions

Oxylet metabolic analyzer 

Location : Building A, Semi-SPF Analysis Room 5

How to use

1. Turn ON the PC monitor power.
2. Turn ON the power for all four Physiocage Units (LE1305).
3. Turn ON the power for the Air Supply Unit (LE400).
4. Turn ON the power for the Gas Analyser Unit (LE405).
5. Run the metabolism program on the PC.
6. Once all equipment is recognized by the Metabolism program, change the Switching time of the Air Supply Unit (LE400) to your desired time. (Default setting: 3 minutes)

Equipment Inquiries : Equipment Center Representative 02-3290-3954

Manual